OECD 427/428: Skin absorption (in vivo and in vitro)
In vivo method
The OECD 427 Skin Absorption in vivo method determines the capacity of a substance to penetrate skin. Usually the research model's skin is dosed with the chemical at a level comparable to expected human exposure levels. Observations are conducted for a duration comparable to expected human exposure duration (often 6 or 24 hours), and toxic effects and blood draws are analyzed and documented.
In vitro method
The OECD 428 Skin Absorption in vitro method tests for the capacity of a skin sample to absorb a test substance. Skin samples from humans or research models are placed in separate chambers of either a static or flow-through system. Temperature is held constant and the absorption process is observed. If applicable, metabolites associated with the absorption process may also be studied.